view profile for Anup Singh

Anup Singh

Director of Microfluidics

view profile for Jess Sustarich

Jess Sustarich

Deputy Director of Microfluidics

view profile for William Gaillard

William Gaillard

Post Doctoral Researcher

Enzymes are the workhorse catalysts involved in many aspects of bioenergy research at JBEI. The Microfluidics Group is developing high-throughput and high-content assay platforms for characterizing enzymes and enzyme-variants, along with their substrates and products. This involves utilization of state-of-the-art microfluidic chips that can be interfaced with fluorescence detectors for rapid and sensitive detection. The goal is to develop cross-cutting microscale technologies for advancing biofuels development that are robust yet easy to use.


  • Development of microfluidic (i.e. lab-on-a-chip) tools for screening ionic liquid and synthetic biology applications.
  • Assay development at the interface of droplet microfluidics and mass spectrometry to streamline the discovery and optimization of biomanufacturing pathways.
  • Development of novel surfactants for microfluidic droplet formation. These sugar based surfactants have diverse biological applications including cell cultures, enzymatic activity assays, DNA synthesis etc.
  • Development of a method for Next-Generation DNA Synthesis.
  • Automation and scale-down of established assays for feedstock deconstruction and fuel synthesis using solids- and liquid-handling robots, with custom hardware for elevated temperatures and evaporation minimization.
  • Adapt electrophoretic glycan analysis to a commercial microfluidic instrument and demonstrate screening of GT and GH enzymes in 96-well format.

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