Anup Singh is the manager of Sandia National Laboratories’ Biosystems Research Department. His expertise is in biotoxin recognition, enzyme-based sensor development and proteoliposomes.
His group’s aim is to create specialized technologies to support the scientific research in feedstock development, biomass degradation, and fuel conversion for efficient conversion of biomass to biofuels. These technologies include high-throughput approaches for surveying enzyme diversity and screening of thousands of natural and modified enzyme variants.
Microfluidic and Array-based assays
- Adapt electrophoretic glycan analysis to a commercial microfluidic instrument and demonstrate screening of GT and GH enzymes in 96-well format.
- Develop a droplet microfluidic system for highly-parallel analysis. Demonstrate in this platform a) screening of multiple cellulose cocktails using insoluble biomass with Deconstruction and b) assembly of genes using multiple cloning methods with Fuels Synthesis.
- “A droplet-to-digital (D2D) microfluidic device for single cell assays”, Lab Chip (2015)
- “A Versatile Microfluidic Device for Automating Synthetic Biology”, ACS Synth Biol (2015)
- “Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems”, Lab Chip (2014)
- “Versatile on-demand droplet generation for controlled encapsulation”, Biomicrofluidics (2014)
- “Rapid Kinetic Characterization of Glycosyl Hydrolases Based on Oxime Derivatization and Nanostructure-Initiator Mass Spectrometry (NIMS)”, ACS Chem Biol (2014)
- “One-pot, microscale cell-free enzyme expression and screening”, Methods Mol Biol (2014)
- “Droplet-based microfluidic platform for heterogeneous enzymatic assays.”, Lab Chip (2013)
- “A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres.”, Chem Communications (2013)
- “Acoustic deposition with NIMS as a high-throughput enzyme activity assay”, Analytical and Bioanalytical Chemistry (2012)
- ” Nanostructure-initiator mass spectrometry (NIMS) for the analysis of enzyme activities”, Curr protoc chem biol (2012)
- “Encoding substrates with mass tags to resolve stereospecific reactions using Nimzyme”, Rapid Communications in Mass Spectrometry (2012)
- “High-throughput enzymatic hydrolysis of lignocellulosic biomass via in-situ regeneration”, Bioresource Technology (2012)
- “Colloid-based multiplexed screening for plant biomass-degrading glycoside hydrolase activities in microbial communities”, Energy and Environmental Science (2011)
- “Microfluidic Glycosyl Hydrolase Screening for Biomass-to-Biofuel Conversion”, Analytical Chemistry (2010)
- “A Microscale Platform For Integrated Cell-Free Expression And Activity Screening Of Cellulases”, Journal of Proteome Research (2010)
- “Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels”, Molecules (2010)
- “Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol”, Molecules (2010)
- “Spectroscopic Analyses of the Biofuels-Critical Phytochemical Coniferyl Alcohol and Its Enzyme-Catalyzed Oxidation Products”, Molecules (2009)