Relationship of halo-tolerance to IL-tolerance

cbh1fromhaloutah
Zhang et al. (Zhang et al.): Hu-CBH1 remains active in high concentrations of several ILs.  Hu-CBH1 was incubated with CMC substrate at 37 ◦C for 1 h in the presence of 2 M NaCl and 10 mM Tris-HCl (pH 7.0), with or without the addition of 20% of [C2C1Im][OAc],[C2C1Im]Cl, [Bmim]Cl or 20%, 30% and 40% of [Amim]Cl. The activities in reactions without ILs were set as 100% for both enzymes.