Technology Data

Access structural information, both raw diffraction data and PDB entries, for proteins that could be optimized to improve the production of advanced biofuels.

(Please note: The 4EWP, 3MMW, and 3EZ8 raw diffraction datasets consist of 180 images approximately 8 MB in size; 3 MMU has 270 images of the same size. 3U48, 3U4A, 3SAE, 3SDR, 3SDT, 3SDU, and 3SDV datasets contain approximately 360 images that are 19 MB; 3SDQ has 720 images, 19 MB each.)

Structural Biology FabH Diffraction Data

The crystal structure of the enzyme FabH (4EWP) from the bacterium, Micrococcus luteus, provides evidence for its catalyzing action during the first step of fatty acid biosynthesis. This pathway has gained attention because fatty acid molecules are favorable precursors for compounds that could substitute for petroleum-derived fuels. Raw diffraction images for this FabH structure can be accessed here. Pereira JH, et al., 2012, contains more information on the activity and selectivity of this molecule.

Structural Biology Cel5A Diffraction Data

Thermatoga maritima Cel5A is a thermally stable glycoside hydrolase and is an excellent candidate for use in the degradation of polysaccharides present on biomass. The Tm_Cel5A structural information (PDB IDs 3MMU and 3MMW) can be used to potentially increase the thermostability of mesophilic cellulase enzymes. Raw diffraction datasets are available for both 3MMU and 3MMW. Refer to Pereira JH, et al., 2010, for further details.

Structural Biology Cel9A Diffraction Data

Endoglucanase Cel9A from the heat- and acid-loving bacteria, Alicyclobacillus acidocaldarius, is used to break down ?-1,4-linked glucans in cellulose. The crystal structure (3EZ8) provides insight into the catalytic mechanism, which could help to optimize process of degrading biomass and the producing of sugars for subsequent fermentation to fuel. Raw diffraction datasets are available for download. Further information can be found in Pereira JH, et al., 2009.

Structural Biology Glucosidase Diffraction Data

A microbial enzyme that could be useful in the pretreatment process of second-generation biofuels was identified from metagenomic analysis of a switchgrass-adapted compost community. The crystal structure of this 3-?-glucosidase was solved in complex with glucose (3U48) and a variant was crystallized with cellopentaose (3U4A). See McAndrew RP, et al., 2013, for a description of this enzyme’s novel structure. Download the raw diffraction datasets here: 3U48, 3U4A.

Structural Biology AgBIS Diffraction Data

The sesquiterpene bisabolene is a precursor to bisabolane, an advanced biofuel. Abies grandis alpha-bisabolene (AgBIS) was used to produce high-titer microbial bisabolene. This sesquiterpene synthase was crystallized in the apo form (3SDQ) and with 5 inhibitors (3SAE, 3SDR, 3SDT, 3SDU, 3SDV). Learn more about this enzyme structure in McAndrew RP, et al., 2011. or by accessing the raw diffraction images (3 SDQ, 3SAE, 3SDR, 3SDT, 3SDU, 3SDV).

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JBEI Targeted Proteomic Methods and Data

Targeted proteomic methods and data for JBEI articles can be found on the Panorama Repository Software for Targeted Proteomics Assays from Skyline.