The primary research goal of the JBEI Feedstocks Division is to generate basic knowledge of plant cell wall biosynthesis to facilitate the engineering of a new generation of robust and high-yielding feedstocks that are sugar enriched, less recalcitrant to deconstruction and generate fewer inhibitors for fermentation.
- Characterization and discovery of key components controlling matrix polysaccharide biosynthesis in rice and Arabidopsis
- Manipulation of lignin and hemicellulose biosynthesis and composition to reduce recalcitrance, eliminate inhibitors of fermentation, and increase the content of the most desirable fuel precursors.
- Development of tools to transfer biomass traits between different species and to stack biomass traits.
Cell Wall Biosynthesis Research Group
- Structure and function of glycosyltransferases, nucleotide sugar transporters and regulatory proteins involved in biosynthesis of hemicellulose, pectin and other glycans.
- Engineering of plants with modified polysaccharide composition and increased content of easily fermentable sugars.
- The impact of cell wall composition on interactions between plants and microbes and on plant stress tolerance.
How Sweet It Is: New Tool for Characterizing Plant Sugar Transporters Developed at Joint BioEnergy Institute
Making Do with More: Joint BioEnergy Institute Researchers Engineer Plant Cell Walls to Boost Sugar Yields for Biofuels
- “Cell Wall Composition and Candidate Biosynthesis Gene Expression Across Rice Development.” Plant Cell Physiol (2016)
- “The Arabidopsis Golgi-localized GDP-fucose transporter is required for plant development.” Nat Commun (2016)
- “Defective Pollen Wall 2 (DPW2) Encodes an Acyl Transferase Required for Rice Male Reproduction.” Plant Physiol (2016)
- “Phosphorylation of a NAC transcription factor by ZmCCaMK regulates abscisic acid-induced antioxidant defense in maize.” Plant Physiol (2016)
- “Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance.” Biotechnol Biofuels (2016)
- “A DUF-246 family glycosyltransferase-like protein affects male fertility and the biosynthesis of rhamnogalacturonan-I arabinogalactans.” BMC Plant Biol (2016)
- “Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls.” Frontiers Plant Sci (2015)
- “MUCI10 produces galactoglucomannan that maintains pectin and cellulose architecture in Arabidopsis seed mucilage.” Plant Physiol (2015)
- “Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses.” Frontiers Plant Sci (2015)
- “Engineering of plant cell walls for enhanced biofuel production.” Curr Opinion Plant Biol (2015)
- “Identification and characterization of the Golgi localized UDP-xylose transporter family from Arabidopsis.” Plant Cell (2015)
- “Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls.” Plant Biotechnol J (2015)
- “A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions in the Golgi apparatus in plants.” J Exp Bot (2015)
- “Complex regulation of Prolyl-4-hydroxylases impacts root hair expansion.” Mol Plant (2015)
- “A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis.” BMC Plant Biol (2014)
- Site-Directed Mutagenesis of IRX9, IRX9L and IRX14 Proteins Involved in Xylan Biosynthesis: Glycosyltransferase Activity is not Required for IRX9 Function in Arabidopsis.”Plos One (2014)
- “Identification of a Sphingolipid ?-Glucuronosyltransferase that is Essential for Pollen Function in Arabidopsis.” Plant Cell (2014)
- “The Golgi UDP-Rhamnose / UDP-Galactose transporter family in Arabidopsis.” Proc Natl Acad Sci USA (2014)
- Xylan biosynthesis.” Current Opinion in Biotechnology (2014)
- “RWA proteins play vital and distinct roles in cell wall O-acetylation in Arabidopsis thaliana.” Plant Physiol (2013)
- “Arabinosylation of a Yariv precipitable cell wall polymer impacts plant growth as exemplified by the arabidopsis glycosyltransferase mutant ray1.” Mol Plant (2013)
- “Golgi-localized enzyme complexes for plant cell wall biosynthesis.” Trends Plant Sci (2013)
- “Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases.” Plant Physiol (2012)
- “Pectin Biosynthesis: GALS1 in Arabidopsis thaliana is a ?-1,4-galactan ?-1,4-galactosyltransferase.” Plant Cell (2012)
- “Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants.” Biotechnol Biofuels (2012)
- “XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan.” Proc Natl Acad Sci USA (2012)