Researchers in the Biofuels Pathways Group discover naturally occurring enzymes that, when integrated with metabolic pathways for biofuel precursors (such as fatty acids), enable engineered microbes to synthesize advanced biofuels. A genome-enabled approach is used to study both pure bacterial cultures and natural microbial communities known to produce the biofuels of interest. In addition to mining existing pathways from nature, researchers in the Biofuels Pathways Group create new pathways by assembling combinations of known genes into non-native pathways to produce fuels optimized for a particular application.
- Development and optimization of pathways for diesel-range methyl ketone biosynthesis in E. coli
- Identification of enzymes involved in biosynthesis of ladderane fatty acids by anammox bacteria
Harry Beller, Director of Biofuels Pathways was one of the interviewees of Chemical & Engineering News cover story about JBEI.
JBEI Researchers Boost Methyl Ketone Production in E. coli
JBEI Researchers Develop a New Candidate for a Cleaner, Greener and Renewable Diesel Fuel
- “Investigation of proposed ladderane biosynthetic genes from anammox bacteria by heterologous expression in E. coli”, PLoS ONE (2016)
- “Natural products as biofuels and bio-based chemicals: fatty acids and isoprenoids”, Natural Product Reports (2015)
- “Biochemical and Structural Studies of NADH-Dependent FabG Used to Increase the Bacterial Production of Fatty Acids under Anaerobic Conditions.” Applied and Environmental Microbiology. (2014)
- “Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies”, Metabolic Engineering (2014)
- “Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones”, Appl. Environ. Microbiol. (2013)
- “Structure of FabH and factors affecting the distribution of branched fatty acids inMicrococcus luteus“, Acta Crystallographica (2012)
- “Engineering of bacterial methyl ketone synthesis for biofuels”, Appl. Environ. Microbiol. (2012)
- “Genes involved in long-chain alkene biosynthesis in Micrococcus luteus“, Appl. Environ. Microbiol. (2010)