Understanding how microbial communities metabolize lignocellulose and identify the genes, pumps and proteins responsible for this activity.
- Collect initial samples from hypersaline environments, determine phylogenetic and functional gene repertoires, and sequence metagenomes
- Develop methods to measure oligosaccharide products of thermophilic cocktails
- Functional screening of expression libraries for genes involved in lignocellulose deconstruction and IL tolerance.
- Develop bioinformatics tools for comparative metagenome analysis
- Establish methods for assaying lignin depolymerization by microbial communities and isolates
JBEI researchers developed MaxBin to automatically recover individual genomes from metagenomes using an expectation-maximization algorithm.
Berkeley Lab to Receive $8.6 Million in Recovery Act Funding for “Transformational” Energy Research Projects
- “Bacillus coagulans tolerance to 1-ethyl-3-methylimidazolium-based ionic liquids in aqueous and solid-state thermophilic culture.”, Biotechnology Progress (2014).
- “Proteogenomic Analysis of a Thermophilic Bacterial Consortium Adapted to Deconstruct Switchgrass”, PLoS One (2013).
- “Community Dynamics of Cellulose-Adapted Thermophilic Bacterial Consortia”, Environmental Microbiology (2013).
- “Thermoascus aurantiacus is a promising source of enzymes for deconstruction of lignocellulosic biomass under thermophilic conditions”, Biotechnology for Biofuels (2012).
- “Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass”, Applied and Environmental Microbiology (2011).